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:[目的]探讨p16、cyclinD1 在胃肠道肿瘤发生、分化中的变化规律。[方法]病人430例 ,用原位杂交技术观察不同病变状态胃肠道粘膜 (正常粘膜、慢性炎症、癌前病变及肿瘤 )中p16、cyclinD1 的基因表达。[结果]正常胃、结肠粘膜p16阳性表达率为90.9%、86% ,cyclinD1 均为阴性 ,萎缩性胃炎、不典型增生、胃癌中p16阳性率分别为78.6%、37.5 %、26.25% ,cyclinD1 阳性率依次为21%、58 %、73.75 % (P<0.05) ;p16与cyclinD1 有交叉共存现象 ,胃癌30% ,结肠癌26.7% ;同时 ,有反向表达现象 ,胃癌占70% ,结肠癌为73.8% ;p16阳性表达率与cyclinD1 的阳性表达率呈显著负相关。[结论]p16的缺失、cyclinD1 的激活 ,可单独或协同促进胃肠肿瘤的发生、发展 ;两者的反向表达趋势提示两者可能存在相互抑制机制。
: [Objective] To investigate the changes of p16 and cyclinD1 in the occurrence and differentiation of gastrointestinal tumors. [Methods] 430 patients were used to observe the gene expression of p16 and cyclinD1 in gastrointestinal tract mucosa (normal mucosa, chronic inflammation, precancerous lesions, and tumors) with different lesions by in situ hybridization. [Results] The positive expression rate of p16 in normal gastric and colonic mucosa was 90.9% and 86%. CyclinD1 was negative. The positive rates of p16 in atrophic gastritis, atypical hyperplasia and gastric cancer were 78.6%, 37.5 % and 26.25 % respectively. CyclinD1 was positive. The rates were 21%, 58%, and 73.75%, respectively (P<0.05). There was a cross-coexistence of p16 and cyclinD1, with 30% of gastric cancer and 26.7% of colon cancer. At the same time, there was a phenomenon of reversed expression. Gastric cancer accounted for 70%. Colon cancer was 73.8%; The positive expression rate of p16 was significantly negatively correlated with the positive expression rate of cyclinD1. [Conclusion] The deletion of p16 and the activation of cyclinD1 can promote the occurrence and development of gastrointestinal tumors either alone or in combination. The reverse expression trend of both suggests that there may be a mutual inhibitory mechanism.