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目的 研究六放珊瑚来源的真菌Exserohilum sp.中活性天然产物.方法 综合运用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备型HPLC等分析和分离技术分离纯化天然产物,利用核磁、质谱等现代波谱分析方法对化合物进行结构鉴定,以致病真茵、细菌和海洋污损细菌为活性模型对化合物进行抗微生物活性评价.结果 从Exserohilum sp.中分离鉴定了4个天然产物(1～4),化合物3对致病真菌Candida albicans和海洋污损细菌Pseudomonas aeruginosa显示中等抑制活性,MIC分别为16.3和32.6 μmol/L.结论 从海洋来源的真菌中分离获得天然产物1～4,化合物3对致病真菌和海洋污损细菌显示中等抑制活性.“,”Objective To isolate bioactive natural products from the zoanthid-derived fungus Exserohilum sp.Methods Silica gel column chromatography (CC),Sephadex LH-20 gel CC,and semi-preparative HPLC were used to isolate and purify the products.NMR and mass spectrum technology were used to confirm the structures of natural products.These natural products were tested for their antimicrobial activities against pathogenic fungi,bacteria,and marine fouling bacteria.Results Four natural compounds (1～4) were isolated from the fungus Exserohilum sp.Compound 3 showed moderate antimicrobial activity against the fungus Candida albicans (MIC=16.3 μmol/L) and the marine fouling bacterium Pseudomonas aeruginosa (MIC=32.6 μmol/L).Conclusion Bioactive natural products 1～4 were isolated from marine-derived fungus Exserohilum sp.,with compound 3 displayed antimicrobial activity against pathogenic fungi and marine fouling bacteria.