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目的探讨氟喹诺酮药物对眼部分离的表皮葡萄球菌的防耐药突变浓度(MPC),并比较MPC与最低抑菌浓度(MIC)的关系。方法采用标准琼脂二倍稀释法测定表皮葡萄球菌对环丙沙星(CIP)、氧氟沙星(OFL)、左氧氟沙星(LVF)及加替沙星(GTF)的MIC值。挑选血液琼脂培养基上表皮葡萄球菌菌落,置于MullerHinton肉汤(MH)中增菌,菌液浓度至1×108cfu/ml,1∶10稀释,每种药每个浓度MullerHinton琼脂培养基(MHA)上接种1μl菌液,菌接种量104cfu。采用混菌法测定MPC值。以上述各种药物对该菌群的MIC50为参考,选择测定MPC时的药物浓度。每平皿中加入1ml药液和19ml细菌与MHA的混悬液,最终菌接种量约1010cfu。结果GTF的MIC90最低,为0.25mg/L;LVF次之,为0.50mg/L;CIP和OFLMIC90相等,为1.00mg/L。GTF对表皮葡萄球菌的MPC50和MPC90最低,分别是1.00mg/L和2.00mg/L;其次是LVF和CIP,均分别为2.00mg/L和4.00mg/L;OFL的MPC50和MPC90分别是2.00mg/L和8.00mg/L。结论LVF和GTF不仅抗菌作用强,而且在预防表皮葡萄球菌耐药突变方面有着明显的优势。(中华眼科杂志,2006,42989-991)
Objective To investigate the anti-drug-resistant mutant concentration (MPC) of fluoroquinolone against Staphylococcus epidermidis isolated from the eye and to compare the MPC with the minimum inhibitory concentration (MIC). Methods MIC of Staphylococcus epidermidis to ciprofloxacin (CIP), ofloxacin (OFL), levofloxacin (LVF) and gatifloxacin (GTF) was determined by standard agar double dilution method. Staphylococcus epidermidis colonies were selected on blood agar medium and placed in Muller Hinton broth (MH) for enrichment. The bacterial concentration was 1 × 10 8 cfu / ml and diluted 1:10. Each concentration of Muller Hinton agar medium (MHA ) On the inoculation 1μl bacteria, inoculation amount of 104cfu. The mixed method was used to determine the MPC value. MIC50 for the above various drugs on the flora as a reference, select the determination of MPC drug concentration. Add 1 ml of liquid and 19 ml of suspension of bacteria and MHA to each dish. The final inoculum size is about 1010 cfu. Results The lowest MIC90 of GTF was 0.25 mg / L, followed by LVF was 0.50 mg / L, and CIP and OFLMIC90 were equal to 1.00 mg / L. GTF had the lowest MPC50 and MPC90 against Staphylococcus epidermidis, 1.00mg / L and 2.00mg / L respectively; followed by LVF and CIP, which were 2.00mg / L and 4.00mg / L, respectively; OFL’s MPC50 and MPC90 were 2.00 mg / L and 8.00 mg / L. Conclusion LVF and GTF not only have strong antibacterial activity, but also have obvious advantages in preventing drug-resistant mutations of Staphylococcus epidermidis. (Chinese Journal of Ophthalmology, 2006,42989-991)