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目的研究Yes相关蛋白1(YAP1)在非小细胞肺癌细胞增殖、细胞周期、迁移、侵袭中的作用。方法通过慢病毒介导的小干扰RNA靶向抑制人非小细胞肺癌细胞株A549中YAP1基因的表达,采用CCK-8法、流式细胞术分别检测沉默YAP1对A549细胞增殖及凋亡周期的影响,Transwell实验观察沉默YAP1对A549细胞迁移、侵袭能力的影响。结果沉默YAP1后,A549细胞YAP1mRNA和蛋白表达均下调(P<0.01),CCK-8实验结果显示沉默YAP1抑制A549的增殖、增加G0/G1期的细胞比例(P<0.01),Transwell实验结果示沉默YAP1抑制A549细胞的迁移、侵袭(P<0.01)。结论沉默YAP1基因对非小细胞肺癌细胞的恶性生物学特征具有抑制作用,YAP1可能成为肺癌治疗的潜在靶标。
Objective To investigate the role of Yes-associated protein 1 (YAP1) in the proliferation, cell cycle, migration and invasion of non-small cell lung cancer. Methods Lentivirus-targeted small interfering RNA targeting inhibited the expression of YAP1 in human non-small cell lung cancer cell line A549. CCK-8 assay and flow cytometry were used to detect the proliferation and apoptosis of A549 cells Effect, Transwell experiments observed silencing YAP1 A549 cell migration, invasion ability. Results After silencing YAP1, the expression of YAP1 mRNA and protein were down-regulated in A549 cells (P <0.01). The results of CCK-8 assay showed that silencing YAP1 could inhibit the proliferation of A549 cells and increase the proportion of cells in G0 / G1 phase (P <0.01) Silencing YAP1 inhibited A549 cell migration and invasion (P <0.01). Conclusion Silencing YAP1 gene can inhibit the malignant biological characteristics of non-small cell lung cancer cells, and YAP1 may be a potential target for the treatment of lung cancer.