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目的建立同时测定保健食品中的红景天苷和腺苷含量的高效液相色谱测定方法。方法采用50%甲醇为溶剂,超声提取样品中的的红景天苷和腺苷成分。采用shim-pack CLS-ODS色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇+20 mmol/L磷酸二氢钾水溶液(15+85,V/V),流速1.0 ml/min,进样量10μl,紫外检测波长为红景天苷275 nm,腺苷260 nm。结果红景天苷在10.0～200μg/ml范围内线性良好(r=0.999 5),平均回收率92.5%～99.5%,精密度2.8%～4.7%(n=6);腺苷在2.50～50.0μg/ml范围内线性良好(r=0.999 6),平均回收率90.0%～94.0%,精密度2.1%～5.2%(n=6)。红景天苷和腺苷检出限LOD分别为0.01和0.005μg/g。结论本方法快捷、简便、灵敏度高,可准确同时测定保健食品中的红景天苷和腺苷含量。
Objective To establish a method for simultaneous determination of salidroside and adenosine in health food by high performance liquid chromatography. Methods Salidroside and adenosine were extracted by sonication using 50% methanol as solvent. A shim-pack CLS-ODS column (150 mm × 4.6 mm, 5 μm) was used with a mobile phase of methanol + 20 mmol / L aqueous potassium dihydrogen phosphate (15 + 85, V / V) Sample volume 10μl, UV detection wavelength of salidroside 275 nm, adenosine 260 nm. Results Salidroside had a good linearity (r = 0.999 5) within the range of 10.0 ~ 200 μg / ml with an average recovery of 92.5% ~ 99.5% and a precision of 2.8% ~ 4.7% The average recovery was 90.0% ~ 94.0% and the precision was 2.1% -5.2% (n = 6). The detection limits of salidroside and adenosine were 0.01 and 0.005 μg / g, respectively. Conclusion The method is fast, simple and sensitive, and can simultaneously determine the content of salidroside and adenosine in health foods.