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γδ T细胞和αβT细胞是两种表型和功能不同的T淋巴细胞亚群。为研究两种细胞在抗原特异性免疫应答中是否具有协同作用,并进一步探讨其相互作用的方式,本文利用实验性自身免疫性葡萄膜炎小鼠模型获得脾脏和淋巴结T细胞,用γδ TCR和αβTCR单克隆抗体染色,分别用带有磁珠的抗FITC和抗PE单克隆抗体通过阳性或阴性选择获得高度纯化的γδ T细胞和αβ T细胞,观察不同比例的γδT细胞和αβT细胞在体外共孵育后IRBP1-20抗原特异性增殖和细胞因子表达,并用细胞培养薄膜将二者分开,测定细胞因子表达情况。结果表明,γδ T细胞或αβT细胞(99%纯度)都不能单独发生抗原特异性免疫应答。只有在二者比例为5%γδT细胞和95%αβT细胞时能产生最大的抗原特异性反应。当两种细胞脱离接触时,不能出现抗原特异性反应。γδ T细胞或αβ T细胞的培养上清也不能使γδT细胞或αβT细胞发生抗原特异性反应。本研究提示,小鼠γδT细胞和αβT细胞在抗原特异性免疫应答中具有协同作用,这种协同作用是通过两种细胞相互接触,γδT细胞协助αβT细胞产生免疫应答实现的。
γδ T cells and αβ T cells are two subpopulations of T lymphocytes that differ in phenotype and function. In order to study whether the two cells have synergistic effect in antigen-specific immune response and to explore the way of their interaction, spleen and lymph node T cells were obtained by using experimental autoimmune uveitis mouse model, αβTCR monoclonal antibody staining, respectively, with anti-FITC with magnetic beads and anti-PE monoclonal antibody by positive or negative selection to obtain highly purified γδ T cells and αβ T cells were observed in different proportions of γδ T cells and αβ T cells in vitro were IRBP1-20 antigen-specific proliferation and cytokine expression after incubation, and the two were separated using a cell culture membrane to measure cytokine expression. The results showed that neither γδ T cells nor αβ T cells (99% pure) were able to undergo antigen-specific immune responses alone. Only in the ratio of 5% γδ T cells and 95% αβ T cells can produce the largest antigen-specific response. When both cells disengage, no antigen-specific reaction can occur. The culture supernatant of [gamma] [delta] T cells or [alpha] [beta] T cells also failed to cause antigen-specific reaction of [gamma] [delta] T cells or [alpha] [beta] T cells. This study suggests that mouse γδT cells and αβT cells have a synergistic effect in antigen-specific immune response. This synergistic effect is achieved by the interaction of the two cells, and the γδT cells help the αβT cells to produce an immune response.