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目的 :了解中国人群上皮源性恶性肿瘤患者 p16基因甲基化异常状况。方法 :收集 131例肺、食管、卵巢、子宫内膜、膀胱、喉、鼻咽癌患者肿瘤组织 ,甲基化敏感限制性内切酶 Sma I消化 DNA,PCR扩增 p16基因外显子 1,分析 5′Cp G岛异常甲基化。结果 :131例患者中 p16基因甲基化异常检出率为 19.1%。甲基化频率较高的肿瘤类型是鼻咽癌 (2 7.9% )、膀胱癌 (2 5 .0 % )和肺癌 (2 5 .0 % )。结论 :在中国人群中上皮源性恶性肿瘤患者中 ,p16基因异常甲基化是较为频繁发生的基因变化 ,可能与病理分级和预后有关。
Objective: To understand the abnormal status of p16 gene methylation in epithelial-derived malignant tumors in Chinese population. Methods: 131 cases of lung, esophagus, ovary, endometrial, bladder, larynx, and nasopharyngeal carcinomas were collected from the tumor tissue, and the methylation-sensitive restriction endonuclease Sma I digestion DNA was used to amplify exon 1 of p16 gene by PCR. Aberrant methylation of 5’Cp G islands was analyzed. Results : The abnormal detection rate of p16 gene methylation in the 131 patients was 19.1%. The types of tumors with higher methylation frequencies were nasopharyngeal carcinoma (2 7.9%), bladder cancer (25.0%), and lung cancer (25.0%). Conclusion : In patients with epithelial-derived malignant tumors in Chinese population, aberrant methylation of p16 gene is a frequently occurring genetic change that may be related to pathological grade and prognosis.