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目的:以人卵巢癌细胞SKOV3为研究对象,探讨Bcl-2抑制剂S1诱导人卵巢癌细胞凋亡的机制。方法:通过MTT法检测S1作用下卵巢癌细胞的生存率;S1 10μmol/L作用不同时间,流式细胞术检测细胞凋亡率;Western Blot检测凋亡相关蛋白Mcl-1的变化。结果:与对照组相比S1能明显抑制SKOV3的生存率;流式细胞术结果显示,S1引起SKOV3细胞凋亡明显升高;蛋白水平检测S1作用8 h能明显抑制SKOV3细胞Mcl-1蛋白表达。结论:S1可能通过抑制Mcl-1蛋白诱导人卵巢癌细胞凋亡。
OBJECTIVE: To investigate the mechanism of Bcl-2 inhibitor S1-induced apoptosis in human ovarian cancer cell line SKOV3. Methods: The survival rate of ovarian cancer cells was detected by MTT assay. The apoptotic rate of S180 cells was detected by flow cytometry at different time points of S1 10μmol / L. The expression of apoptosis related protein Mcl-1 was detected by Western Blot. Results: Compared with the control group, S1 could significantly inhibit the survival rate of SKOV3 cells. The results of flow cytometry showed that the apoptosis of SKOV3 cells was significantly increased by S1, and the expression of Mcl-1 protein in SKOV3 cells was significantly inhibited by S1 . Conclusion: S1 may induce apoptosis of human ovarian cancer cells by inhibiting Mcl-1 protein.