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目的探讨白藜芦醇(resveratrol,RES)改善重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠胰腺微循环的作用机制。方法SD大鼠随机分为3组:假手术(shamoperation,SO)组、重症急性胰腺炎(SAP)模型组、白藜芦醇治疗(RES)组。以40g/L牛黄胆酸钠0.1mL/100g胆胰管逆行注射建立SAP模型。RES组术后立即经阴茎背静脉注射RES(20mg/kg)。分别于术后3、6、12h取材,进行胰腺组织学评分;检测胰腺组织环氧化酶2(cyclcoxygenase-2,COX-2)、诱生型一氧化氮合酶(inducible nitric oxide synthase,i NOS)的表达和一氧化氮(nitrogen monoxide,NO)含量。结果RES组胰腺损伤较SAP组明显减轻(P<0.05)。SAP组COX-2、i NOS的表达和NO含量在各时间点均显著高于假手术组(P<0.05);RES组COX-2的表达在6、12h较SAP组明显下降,i NOS和NO在各时间点均较SAP组明显下降,差异具有统计学意义(P<0.05)。结论白藜芦醇可能通过下调COX-2和i NOS的表达改善胰腺微循环。
Objective To investigate the mechanism of resveratrol (RES) on pancreatic microcirculation in rats with severe acute pancreatitis (SAP). Methods SD rats were randomly divided into 3 groups: sham operation (SO) group, severe acute pancreatitis (SAP) model group and resveratrol treatment group (RES). SAP model was established by retrograde injection of 40g / L sodium taurocholate 0.1mL / 100g into the pancreatic duct. The RES group was injected with RES (20 mg / kg) via the penis dorsal vein immediately after operation. The rats were sacrificed at 3, 6 and 12 h postoperatively respectively for histological evaluation of pancreatic tissue. Cyclooxygenase 2 (COX-2), inducible nitric oxide synthase (i NOS) expression and nitrogen monoxide (NO) content. Results The pancreatic injury in RES group was significantly lower than that in SAP group (P <0.05). The expression of COX-2, iNOS and the content of NO in SAP group were significantly higher than those in sham operation group at each time point (P <0.05). The COX-2 expression in RES group was significantly lower than that in SAP group at 6 and 12h NO at each time point was significantly lower than the SAP group, the difference was statistically significant (P <0.05). Conclusion Resveratrol may improve pancreatic microcirculation by down-regulating the expression of COX-2 and iNOS.