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利用L-谷氨酸苄酯开环聚合得到聚L-谷氨酸苄酯,对其进行表征,将聚L-谷氨酸苄酯溶于四氢呋喃后涂敷在3-氨丙基三乙氧基硅胶上制得液相色谱固定相,研究了正相色谱条件下聚L-谷氨酸苄酯涂敷型固定相对9种位置异构体及10种手性化合物的拆分能力。以不同比例的正己烷/异丙醇为流动相,有6种位置异构体(o,m,p-氯苯胺、o,m,p-溴苯胺、o,m,p-碘苯胺、o,m,p-硝基苯胺、o,m,p-二硝基苯和o,m,p-苯二胺)和4种手性化合物(1-(对氯苯基)乙醇、5-二硝基-N-(1-苯乙基)苯甲酰胺、华法林和四咪唑)得到不同程度的拆分,表明聚L-谷氨酸苄酯涂敷型固定相对位置异构体具有较好的识别作用,同时也表现出良好的手性拆分能力。
L-glutamic acid benzyl ester was subjected to ring-opening polymerization to obtain poly-L-glutamic acid benzyl ester which was characterized. The poly-L-glutamic acid benzyl ester was dissolved in tetrahydrofuran and then coated on 3-aminopropyltriethoxy The stationary phases of liquid chromatography were prepared on silica gel. The resolving power of 9 positional isomers and 10 chiral compounds were studied under normal phase chromatographic conditions. There are six kinds of positional isomers (n, m, p-chloroaniline, o, m, p-bromoaniline, o, m, p-iodoaniline, o , p-nitroaniline, o, m, p-dinitrobenzene and o, m, p-phenylenediamine) and four chiral compounds (1- (p- chlorophenyl) ethanol, Nitro-N- (1-phenylethyl) benzamide, warfarin, and tetraimidazole), indicating that the relative position isomers of the poly-L-glutamic acid benzyl ester- Good recognition, but also shows good chiral resolution.