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目的:建立润燥止痒胶囊中苦参碱和二苯乙烯苷的含量测定方法。方法:采用固相萃取-HPLC,色谱柱为XTerraRP18(3.9 mm×150 mm,5μm),流速1.0 mL·min-1,乙腈-水(20∶80)为流动相,检测波长为320 nm。结果:苦参碱的线性范围为0.302 6～1.513 g·L-1,r=0.999 7,平均回收率为98.65%,RSD 1.90%(n=6);二苯乙烯苷的线性范围为1.048～8.384 mg·L-1,r=0.999 5,平均回收率为97.45%,RSD 1.89%(n=6)。结论:方法简便、快速、准确,适合该胶囊中苦参碱和二苯乙烯苷的含量测定。
Objective: To establish a method for determination of matrine and stilbene glycoside in Runzao Zhiyang capsule. Methods: The mobile phase was acetonitrile-water (20:80) with a flow rate of 1.0 mL · min-1 using XTerraRP18 (3.9 mm × 150 mm, 5 μm) as the mobile phase at a detection wavelength of 320 nm. Results: The linear range of matrine was 0.302 6 ~ 1.513 g · L -1, r = 0.999 7, the average recovery was 98.65%, RSD 1.90% (n = 6). The linear range of stilbene glycosides was 1.048 ~ 8.384 mg · L-1, r = 0.999 5, the average recovery was 97.45%, RSD 1.89% (n = 6). Conclusion: The method is simple, rapid, accurate and suitable for the determination of matrine and stilbene glycosides in this capsule.