论文部分内容阅读
目的：检测６７例胆囊癌、１０例胆囊腺瘤及癌旁粘膜的ｐ５３蛋白表达，分析其与肿瘤组织学类型的关系。方法：应用免疫组化技术。结果：ｐ５３蛋白胆囊癌阳性表达为５９．７％，腺瘤为２０％。阳性细胞主要分布在腺瘤的异型增生区；癌旁粘膜ｐ５３蛋白阳性表达在异型增生上皮２／４例，单纯增生上皮５／１４例，肠化上皮１／２例。腺癌ｐ５３蛋白阳性率Ⅰ级４８．４８％，Ⅱ级９０．９％，Ⅲ级７１．４２％，差异有显著性（Ｐ＜０．０５）；Ⅰ级腺癌中，乳头状腺癌阳性率高达６６．６６％。结论：腺瘤及癌旁粘膜中的异型增生、化生及单纯性增生上皮ｐ５３蛋白过度表达，均有发生恶变的潜在可能。
OBJECTIVE: To detect the expression of p53 protein in 67 cases of gallbladder carcinoma, 10 cases of gallbladder adenoma and paraneoplastic mucosa, and to analyze the relationship between the expression of p53 protein and histological types. Methods: The application of immunohistochemistry. Results: The positive expression of p53 gallbladder carcinoma was 59.7%, and adenoma was 20%. The positive cells were mainly distributed in the dysplasia of adenomas; the para-cancer mucosa p53 protein was positively expressed in 2/4 dysplasia epithelium, 5/14 simple hyperplasia epithelium, and 1/2 intestinal metaplasia. The positive rate of p53 protein in adenocarcinoma was 48.48% in grade I, 90.9% in grade II, and 71.42% in grade III. The difference was statistically significant (P<0.05). Papillary adenocarcinoma was positive in grade I adenocarcinoma. The rate is as high as 66.66%. CONCLUSIONS: The overexpression of p53 protein in dysplasia, metaplasia, and simple hyperplasia of adenomas and paraneoplastic mucosa all have potential for malignant transformation.