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目的观察中药黄斑明目胶囊对N-甲基-N-亚硝脲(MNU)诱导大鼠视网膜变性的早期拮抗效应。方法将鼠龄为43 d的♀SD大鼠30只随机分为3组,每组10只。黄斑明目胶囊组以中药黄斑明目胶囊溶解后灌胃,正常对照组和模型对照组以等量蒸馏水灌胃,bid,连续7 d。给药7d后,黄斑明目胶囊组和模型对照组大鼠予MNU 40 mg·kg~(-1),ip;正常对照组注射等量生理盐水。观察MNU处理后12、24、48、72 h各组大鼠视网膜电图(ERG)a波及b波的变化,并于MNU处理后7 d处死大鼠,取眼球做组织学观察。结果正常对照组大鼠不同时间点ERG a波及b波的振幅差异无统计学意义;模型对照组在MNU处理后ERG a波及b波的振幅均呈持续性、进行性下降;中药黄斑明目胶囊可明显抑制ERG a波及b波振幅的降低。通过透视电子显微镜和光学显微镜对视网膜的组织学观察显示,中药黄斑明目胶囊组的病理组织学损害较模型对照组明显减轻。结论中药黄斑明目胶囊可拮抗MNU诱导的视网膜变性大鼠的早期损伤。
Objective To observe the early antagonism of traditional Chinese medicine Huangmao Mingmu capsule on retinal degeneration induced by N-methyl-N-nitrosourea (MNU) in rats. Methods Thirty Sprague Dawley rats aged 43 days were randomly divided into 3 groups, 10 in each group. Huangmao Mingmu capsule group was treated with Huangshi Mingmu capsules dissolved by gavage. The normal control group and model control group were fed with equal distilled water for 7 days. After 7 days of administration, the rats in the macular eyesight capsule group and the model control group were given MNU 40 mg·kg -1 ip, and the normal control group was injected with the same amount of normal saline. The changes of a-wave and b-wave of electroretinogram (ERG) were observed at 12, 24, 48, and 72 h after MNU treatment. The rats were sacrificed 7 days after MNU treatment. The eyeballs were taken for histological observation. Results There was no significant difference in amplitude of ERG a wave and b wave at different time points in normal control rats. The amplitude of ERG a and b waves in the model control group after MNU treatment was continuous and progressively decreased. Chinese herbal medicine Huangye Mingmu Capsule Can significantly inhibit the ERG a wave and b wave amplitude reduction. Histological observation of the retina by fluoroscopy electron microscope and light microscope showed that the pathological and histological damage of the Huangye Mingmu capsule group was significantly reduced compared with the model control group. Conclusion Huanghuang Mingmu Capsule can antagonize the early damage of MNU-induced retinal degeneration rats.