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[Objective] To investigate the immunogenicity of main antigen epitope of RHDV (Rabbit haemorrhagic disease virus) VP60 expressed in a prokaryotic system. [Method] The major antigen epitope gene of RHDV VP60 was amplified by RT-PCR. It was cloned into pET-28b (+) and expressed in E. coli Rosetta strain. The recombinant protein was detected by Western blot. The purified recombinant protein was used to immunize rabbits in order to observe its immunogenicity. [Result] Western blot analysis revealed a clear band at approximately 24.0 kDa. The purified recombinant protein reacted with the purified RHDV in ELISA. [Conclusion] The prokaryotically expressed main antigen epitope of RHDV VP60 shows good immunogenicity.
[Objective] To investigate the immunogenicity of main antigen epitope of RHDV (Rabbit haemorrhagic disease virus) VP60 expressed in a prokaryotic system. [Method] The major antigen epitope gene of RHDV VP60 was amplified by RT-PCR. It was cloned into pET- 28b (+) and expressed in E. coli Rosetta strain. The recombinant protein was detected by Western blot. The purified recombinant protein was used to immunize rabbits in order to observe its immunogenicity. [Result] Western blot analysis revealed a clear band at approximately 24.0 kDa. The purified recombinant protein reacted with the purified RHDV in ELISA. [Conclusion] The prokaryotically expressed major antigen epitope of RHDV VP60 shows good immunogenicity.