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磷脂酶D(PLD)为植物中重要的一种水解酶,PLD在逆境下不仅参与细胞膜的老化过程和种子萌发的过程,还作为一个信号物质响应脱落酸的形成。为了进一步明确玉米PLDs基因家族的功能,通过对玉米PLDs基因家族基本信息的预测,结构域的预测,染色体定位,基因结构的分析,蛋白系统进化树分析以及盐碱胁迫下的ZmPLDs进行转录组分析,共鉴定出ZmPLDs15个,分布在α、β、ε、ζ四个亚家族中,且预测基因主要功能为响应逆境信号和ABA诱导的气孔开闭过程。通过对ZmPLDs生物信息学分析进一步明确了ZmPLDs基因家族的生物学功能。
Phospholipase D (PLD) is an important hydrolytic enzyme in plants. Under adverse conditions, PLD not only participates in cell membrane aging and seed germination, but also acts as a signaling substance in response to ABA formation. In order to further clarify the function of maize PLDs gene family, the transcriptome analysis was performed by predicting the basic information of PLDs gene family in maize, predicting the domains, chromosome location, gene structure analysis, phylogenetic tree analysis and ZmPLDs under saline-alkali stress A total of 15 ZmPLDs were identified and distributed in four subfamilies of α, β, ε and ζ. The major functions of the predicted genes were response to stress signals and ABA-induced stomatal opening and closing processes. Through the bioinformatics analysis of ZmPLDs, the biological function of ZmPLDs gene family was further clarified.