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采用体外孵育大鼠黄体细胞的方法,观察了β-EP,β-LPH,α-MSH 和 ACTH 等阿黑皮素原(POMC)相关肽对孕酮分泌的影响,结果表明:β-EP(10~(-6),10~7,10~(-6)mol/L)可明显促进黄体细胞基础孕酮分泌,呈剂量-效应依赖关系,孕酮含量分别由对照组36.56±8.63pmol/10~6细胞上升为52.90±18.31(P<0.01),73.86±18.21和80.37±9.86pmol/10~6细胞(P<0.01);β-EP(10~(-7)mol/L)还能加强hCG 致孕酮生成作用,孕酮含量由47.30±11.57上升为63.34±9.32pmol/10~6细胞(P<0.01);纳络酮(10~(-6)mol/L)可完全阻断β-EP 的作用。与β-EP 不同,β-LPH(10~(-6)mol/L)抑制基础和 hCG 诱导的孕酮分泌;α-MSH(10~(-6)mol/L)对基础孕酮分泌无明显影响,但能加强 hCG 致孕酮生成作用;ACTH 对大鼠黄体细胞孕酮分泌无明显影响。实验显示:某些 POMC 相关肽,尤其是β-EP 可能以旁分泌的方式参与黄体细胞孕酮分泌的调节。
The effects of POMC-related peptides such as β-EP, β-LPH, α-MSH and ACTH on the secretion of progesterone were observed by in vitro incubation of rat luteal cells. The results showed that β-EP 10 ~ (-6), 10 ~ 7,10 ~ (-6) mol / L) could significantly promote the secretion of basal progesterone in luteal cells in a dose-dependent manner. The contents of progesterone in the control group were 36.56 ± 8.63pmol / 10 ~ 6 cells increased to 52.90 ± 18.31 (P <0.01), 73.86 ± 18.21 and 80.37 ± 9.86 pmol / 10 ~ 6 cells (P <0.01) The progesterone production increased from 47.30 ± 11.57 to 63.34 ± 9.32 pmol / 10 ~ 6 cells (P <0.01), and naloxone (10 -6 mol / L) could be blocked completely The role of β-EP. Unlike β-EP, β-LPH (10 -6 mol / L) inhibited basal and hCG-induced progesterone secretion; α-MSH (10 -6 mol / L) But significantly enhanced the production of progesterone induced by hCG. ACTH had no significant effect on the progesterone secretion of rat luteal cells. Experiments have shown that certain POMC-related peptides, especially beta-EP, may be involved in the regulation of progesterone secretion in the luteal cells in a paracrine manner.