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目的探讨精子发生过程中dysbindin-1的表达及dysbindin-1对精子顶体形态的影响。方法取生后7d、14d、21d、28d、35d和3月龄的雄性小鼠各3只,用免疫印迹法检测睾丸组织dysbindin-1的表达;以dysbindin-1缺失突变的sdy小鼠为研究对象,收集附睾尾部精子,一部分制备精子涂片,HE染色显示精子形态,用异硫氰酸荧光素-豌豆凝集素(FITC-PSA)和抗精子蛋白56(sp56)单克隆抗体进行荧光染色显示顶体的结构;另一部分采用免疫印迹法检测精子中dysbindin-1的表达。结果不同发育阶段小鼠睾丸组织及精子中均只有dysbindin-1A,无dysbindin-1C的表达;sdy小鼠的精子及顶体的形态未见明显异常。结论 Dysbindin-1A表达于小鼠精子发生的不同时期,dysbindin-1A对精子形态维持不起关键作用。
Objective To investigate the expression of dysbindin-1 during spermatogenesis and the effect of dysbindin-1 on the sperm acrosome morphology. Methods Three male SD rats of 7d, 14d, 21d, 28d, 35d and 3 months after birth were killed. The expression of dysbindin-1 in testis was detected by immunoblotting. The sdy mice with dysbindin-1 deletion mutation Subjects: Epididymal sperm were collected, sperm smear was prepared, and the morphology of sperm was observed by HE staining. Fluorescence staining was performed by using fluorescein isothiocyanate-pea agglutinin (FITC-PSA) and antisperm protein 56 (sp56) monoclonal antibody Acrosome structure. The other part was used to detect the expression of dysbindin-1 in sperm by immunoblotting. Results There were only dysbindin-1A in testes and sperm at different developmental stages, but no dysbindin-1C expression in spermatozoa. The sperm and acrosome morphology of sdy mice showed no obvious abnormalities. Conclusions Dysbindin-1A is expressed at different stages of spermatogenesis in mice. Dysbindin-1A can not maintain the morphology of spermatozoa.