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目的探讨ultraviolet C诱导的旁观者效应的可能机制。方法用辐射强度为9 mJ/cm2 UVC照射V 79细胞20 s,分别在第4、8、12 h取靶细胞辐照条件培养基(ICM)培养正常细胞24 h。四甲基偶氮唑盐法测定细胞存活率;观察染色体畸变;流式细胞仪检测细胞凋亡率。结果随着ICM时段的往后推移,细胞的存活率逐渐上升,4、8、12 h时段ICM培养的细胞存活率分别为(61.5±3.9)%、(78.8±3.3)%、(84.2±4.1)%,与对照组比较,差异均有统计学意义(P<0.01);染色体畸变率下降,4、8、12 h时段染色体畸变率分别为12.33%、8.67%、5.67%;细胞凋亡率逐渐降低,4、8、12 h时段细胞凋亡率分别为(20.78±2.38)%、(13.45±1.99)%、(10.51±1.53)%,与对照组(3.04±0.39)%比较,差异有统计学意义(P<0.01)。结论用照射剂量为180mJ/cm2 UVC处理靶细胞后的ICM能够诱导出明显旁观者效应,其表现为细胞凋亡。
Objective To explore the possible mechanism of bystander effect induced by ultraviolet C. Methods V 79 cells were irradiated with UVC at a radiation intensity of 9 mJ / cm2 for 20 s, and normal cells were cultured in ICM for 24 h on the 4th, 8th and 12th hour, respectively. Cell viability was determined by MTT method. Chromosome aberrations were observed. Flow cytometry was used to detect apoptosis rate. Results The survival rate of ICM cells gradually increased with the ICM time-lapse, and the survival rates of ICM cells cultured for 4, 8 and 12 h were (61.5 ± 3.9)%, (78.8 ± 3.3)% and (84.2 ± 4.1) )%, Compared with the control group, the difference was statistically significant (P <0.01); chromosome aberration rate decreased, 4,8,12 h period chromosome aberration rates were 12.33%, 8.67%, 5.67%; apoptosis rate (20.78 ± 2.38)%, (13.45 ± 1.99)% and (10.51 ± 1.53)% respectively at the 4th, 8th and 12th hour. Compared with the control group (3.04 ± 0.39)%, the difference was Statistical significance (P <0.01). Conclusion The ICM of target cells treated with UVC at a dose of 180mJ / cm2 can induce significant bystander effect, which is manifested as apoptosis.