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目的:采用均匀设计和偏最小二乘回归结合因子分析考察大黄-厚朴中大黄素、芦荟大黄素、厚朴酚及和厚朴酚4种成分的组方配伍规律。方法:健康雄性SD大鼠160只,随机分为8个组,依次为假手术组、模型组及四因素六水平均匀设计的6个配方组。胰胆管内逆行注射3.5%牛磺胆酸钠法建立急性胰腺炎大鼠模型。术后6,24 h分别检测血清淀粉酶(AMS),胰脂肪酶(PL),白细胞介素-6(IL-6),IL-10,肿瘤坏死因子-α(TNF-α)的含量变化。偏最小二乘回归分析探索各因素对各药效指标的影响规律,因子分析抽提多项指标中的公共因子。结果:各组分对急性胰腺炎病症的贡献度排序为厚朴酚>和厚朴酚>大黄素>芦荟大黄素。因子分析抽提出隐藏在多项指标后的公共因子为胰腺损伤因子(AMS,IL-6和TNF-α)和炎症因子(PL和IL-10)。结论:采用均匀试验设计、偏最小二乘回归结合因子分析可有效地分析组分配伍中多因素与多效应成分之间的关联规律,为临床病症的治疗提供方-症更为对应的组分配方。
OBJECTIVE: To investigate the compatibility of four prescriptions of emodin, aloe emodin, honokiol and honokiol in rhubarb - magnolia officinalis by means of even design and partial least squares regression combined with factor analysis. Methods: 160 healthy male Sprague-Dawley rats were randomly divided into 8 groups: sham operation group, model group and six formula groups with four factors and six levels. Rat models of acute pancreatitis were established by retrograde injection of 3.5% sodium taurocholate into pancreatic duct. The levels of serum amylase (AMS), pancreatic lipase (PL), interleukin-6 (IL-6), IL-10 and tumor necrosis factor-α . Partial least-squares regression analysis to explore the impact of various factors on the efficacy of the law, factor analysis extracted a number of indicators of public factors. Results: The contribution of each component to acute pancreatitis was ranked as honokiol> honokiol> emodin> aloe-emodin. Factor analysis extracted common factors implicated in multiple indicators of pancreatic injury factors (AMS, IL-6 and TNF-α) and inflammatory factors (PL and IL-10). Conclusion: The uniform experimental design and partial least-squares regression combined with factor analysis can effectively analyze the correlation between multi-factor and multi-effect components in the compatibility of components and provide more appropriate components for the treatment of clinical conditions formula.