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目的对2006年广州地区食源性疾病中分离的肠炎沙门菌进行分子分型,探讨广州地区肠炎沙门菌的分子型别和多态性,为食源性疾病溯源及致病菌数据库的建立提供依据。方法采用限制性内切酶XbaI,对2006年分离到的菌株进行PFGE分子分型,使用BioNumericsVersion4.0软件(使用Dice系数和UPGMA法)对菌株进行聚类分析,并与深圳市的肠炎沙门菌PFGE型别进行比较。结果所有74株肠炎沙门菌均得到一致的PFGE克隆型,表明两次不同的食源性疾病均由同一PFGE型引起。广州与深圳的肠炎沙门菌PFGE图谱的比较表明,两地食源性疾病分离株具有很近的亲缘关系。结论PFGE分子分型与流行病学资料紧密结合可增强对肠炎沙门菌食源性疾病的溯源和预警。
Objective To analyze the molecular typing of Salmonella enteritidis isolated from food-borne diseases in Guangzhou in 2006 and to explore the molecular types and polymorphisms of Salmonella enteritidis in Guangzhou and to provide a traceable source of foodborne diseases and establishment of a database of pathogens in accordance with. Methods The strains isolated in 2006 were genotyped by PFGE using restriction endonuclease XbaI. The strains were clustered using BioNumerics Version 4.0 software (using Dice coefficient and UPGMA method) and compared with Salmonella enteritidis PFGE type for comparison. Results All 74 Salmonella enteritidis strains had a consistent PFGE clone indicating that two different foodborne diseases were caused by the same PFGE type. Comparison of the PFGE patterns of Salmonella enteritidis between Guangzhou and Shenzhen showed that the isolates of foodborne diseases in both areas had close genetic relationship. Conclusion The close association of PFGE molecular typing and epidemiological data can enhance the traceability and early warning of foodborne diseases of Salmonella enteritidis.