中国黑腹果蝇中分离变相因子[Segregation Distorter(SD)]的初步研究

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黑腹果蝇(Drosophila melanogaster)中的分离变相因子[Segregation Distorter (SD)]是一种非常典型的具有减数分裂驱动性质的特殊遗传因子.SD在世界不同地区的黑腹果蝇群体中广泛存在,通过杂交的方法测得其频率在1%~5%范围之内.首次在中国北京、青岛采集大量野生黑腹果蝇样本,对SD进行频率测定,发现SD在中国野生黑腹果蝇群体中也广泛存在,且频率与世界其他地区SD频率基本吻合.在此基础之上,进一步从北京北安河地区的黑腹果蝇群体中筛选得到一个SD果蝇品系(SD bah).采用了PCR扩增SD片段的方法鉴定SD的存在,与传统的杂交方法相比,此法既简便、快速,又准确、可靠,该方法的建立在SD频率测定、SD品系建立及其他研究中具有较高的应用价值.
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