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目的探讨血管内皮生长因子 C(VEGF-C)在恶性淋巴瘤患者淋巴瘤组织中的表达及其与疾病进展的关系。方法运用实时定量 PCR 方法检测了81例恶性淋巴瘤患者淋巴瘤组织中VEGF-C 的表达。联合激光微切割技术和定量 PCR 法从淋巴瘤组织中特异性分离淋巴瘤细胞,检测淋巴瘤细胞中 VEGF-C 的表达。同时通过电镜对淋巴瘤组织切片中血管结构进行观察。结果与淋巴结反应性增生患者(8例)淋巴结的 VEGF-C 表达量(1.55±0.19)相比,血管免疫母细胞性 T 细胞淋巴瘤患者(18例)组织(15.35±9.07)和微切割的患者(10例)淋巴瘤细胞(15.19±4.28)均高表达VEGF-C(P 值分别为0.0020 和<0.01)。VEGF-C 高表达的患者常伴骨髓浸润(P=0.0039)和皮肤累及(P=0.0046),国际预后指数分组多为高危组(P=0.0302)。VEGF-C 高表达者存在血管结构异常,表现为血管内皮细胞肿胀,或缺乏周围细胞。结论 VEGF-C 表达与血管免疫母细胞性 T 细胞淋巴瘤的疾病进展密切相关。
Objective To investigate the expression of vascular endothelial growth factor C (VEGF-C) in lymphoma of patients with malignant lymphoma and its relationship with disease progression. Methods Real-time quantitative PCR was used to detect the expression of VEGF-C in lymphoma of 81 patients with malignant lymphoma. Combined with laser microdissection and quantitative PCR method, lymphoma cells were exclusively isolated from lymphoma tissues to detect the expression of VEGF-C in lymphoma cells. At the same time, the structure of blood vessels in lymphoma tissue sections was observed by electron microscopy. Results Compared with the expression of VEGF-C in lymph nodes (1.55 ± 0.19) in patients with lymph node reactive hyperplasia (n = 8), the number of patients with angioimmunoblastic T cell lymphoma (n = 15) VEGF-C was highly expressed in all patients (15.19 ± 4.28) (P = 0.0020 and <0.01, respectively). Patients with high VEGF-C expression were often associated with bone marrow infiltration (P = 0.0039) and skin involvement (P = 0.0046). Most of the international prognostic groups were high risk (P = 0.0302). Vascular endothelial dysplasia was observed in patients with high expression of VEGF-C, manifested as swollen vascular endothelial cells, or lack of surrounding cells. Conclusion The expression of VEGF-C is closely related to the progress of the disease of vascular immunoblastic T-cell lymphoma.