论文部分内容阅读
目的:探讨抗核抗体谱(ANA谱)检测和不同方法双链DNA(ds-DNA)检测在系统性红斑狼疮(SLE)诊断中的临床意义。方法:收集162例SLE患者和50例健康对照者的血清,用间接免疫荧光法(I-IF)检测抗核抗体(ANA),用酶联免疫吸附法(ELISA)、欧蒙印迹法和间接免疫荧光法检测抗ds-DNA抗体,用欧蒙印迹法测定抗核抗体谱。结果:SLE患者ANA阳性率为90.3%;ELISA、欧蒙印迹法、IIF检测抗ds-DNA抗体的阳性率分别是25.3%、37.6%、35.8%,抗ds-DNA抗体总体阳性率为49.0%;抗核抗体谱中抗U1-nRNP抗体、抗Sm抗体、抗SS-A抗体、抗SS-B抗体、抗核小体抗体、抗组蛋白抗体、抗核糖体P蛋白检测的阳性率分别是25.3%、9.9%、63.0%、7.4%、20.4%、22.2%、24.1%,均明显高于健康对照组(P<0.05)。结论:ANA谱和ds-DNA检测提高了SLE的检测敏感度和诊断率,可减少SLE的误诊和漏诊情况,对SLE的诊断和治疗有重要的临床意义。
Objective: To investigate the clinical significance of anti-nuclear antibody (ANA) spectrum detection and different methods of double-stranded DNA (ds-DNA) detection in the diagnosis of systemic lupus erythematosus (SLE). Methods: Serum from 162 patients with SLE and 50 healthy controls were collected. Anti-nuclear antibody (ANA) was detected by indirect immunofluorescence assay (ELISA), Western blotting and indirect Anti-ds-DNA antibodies were detected by immunofluorescence and anti-nuclear antibody profiles were determined by Western blotting. Results: The positive rate of ANA in SLE patients was 90.3%. The positive rates of anti-dsDNA antibody in ELISA, Amunot blot and IIF were 25.3%, 37.6% and 35.8%, respectively. The overall positive rate of anti-dsDNA antibody was 49.0% The positive rates of anti-U1-nRNP antibody, anti-Sm antibody, anti-SS-A antibody, anti-SS-B antibody, anti-nucleosome antibody, anti-histone antibody and anti-ribosomal P protein in the antinuclear antibody spectrum were 25.3%, 9.9%, 63.0%, 7.4%, 20.4%, 22.2% and 24.1%, respectively, were significantly higher than those in healthy controls (P <0.05). Conclusion: The detection of ANA and ds-DNA can improve the detection sensitivity and diagnostic rate of SLE, reduce the misdiagnosis and missed diagnosis of SLE, and have important clinical significance for the diagnosis and treatment of SLE.