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目的建立羟亚胺及氯胺酮定性和定量分析方法。方法分别使用气相色谱质谱联用法(GC/MS)、液相色谱质谱联用法(LC/MS)和液相色谱紫外法(LC/UV)分析羟亚胺,考察各方法的特点及适用范围。结果采用GC/MS法分析时,进样口的高温会导致部分羟亚胺转化为氯胺酮。LC/MS及LC/UV分析则不存在干扰,羟亚胺和氯胺酮的线性范围分别为3.0～300 ng/mL(LC/MS)、0.02～1.00mg/mL(LC/UV);最低检测限分别为1.0ng/mL(LC/MS)、5.0μg/mL(LC/UV)。结论 GC/MS法仅可确定样品中羟亚胺的存在,不能确认是否含有氯胺酮。LC/MS和LC/UV法可分别用于痕量和常量羟亚胺和氯胺酮定性和定量分析。
Objective To establish qualitative and quantitative analysis methods of hydroxyimine and ketamine. Methods The hydroxyimine was analyzed by gas chromatography-mass spectrometry (GC / MS), liquid chromatography-mass spectrometry (LC / MS) and liquid chromatography- ultraviolet spectroscopy (LC / UV), respectively. Results When analyzed by GC / MS, the high temperature at the inlet led to the conversion of some of the hydroxyimine into ketamine. LC / MS and LC / UV analysis showed no interference. The linear range of hydroxyimine and ketamine was 3.0-300 ng / mL (LC / MS) and 0.02-1.00 mg / mL (LC / UV) 1.0 ng / mL (LC / MS) and 5.0 μg / mL (LC / UV), respectively. Conclusions GC / MS only confirms the presence of hydroxyimine in the sample and does not confirm the presence or absence of ketamine. LC / MS and LC / UV methods were used for the qualitative and quantitative analysis of traces and constants of hydroxyimine and ketamine, respectively.