OBJECTIVE To study the treatment effect of Gastrodia elata and Uncaria rhynchophylla water extract(GUW)against cerebral ischemia,and the metabolomic profile of cerebrospinal fluid in GUW treated cerebral ischemia in rat model.METHODS Middle cerebral artery occlusion was performed in male SD rat.GUW or control(PBS)was administered orally for 7d post-operationally.At day 7,the brain of the rat was harvested.The infarct volume of the brain was measured after TTC staining.The brain was subjected to H&E and cresyl violet staining in histological analysis.The cerebrospinal fluid(CSF)of another set of animals was aspirated.The CSF samples were labeled with 13 C-dansyl and 12C-dansyl chloride.The reaction mixture was put into liquid chromatography Fourier-transform ion cyclotron resonance mass spectrometry for analysis of the CSF metabolome.Multivariate statistical analysis will be carried out to determine the differences of metabolomic profile in CSF of GUW versus control group.RESULTS After GUW treatment,the neurological deficit score was significantly improved from day 3 to 7(P<0.001).GUW treated group significantly reduced 55.7%infarct volume on MCAO rats in TTC stained brain slices(P<0.001)and preserved brain histological structure observed in H&E and cresyl violet staining.On the other hand,according to various statistical analyses to compare CSF metabolite in the GUW and control group,volcano plot analysis showed 48 metabolites(P<0.001);OPLS-DA analysis showed 129 metabolites(VIP score >2.0)and 45 metabolites in ROC curve analysis.Among identified metabolites,L-aspartic acid(60%)and glycine(62%)decreased in GUW while threoninyl-alanine increased by 1.6 fold significantly.CONCLUSION GUW ameliorated cerebral ischemic injury,and the metabolomic approach may be a potential means to find biomarker of the Chinese medicine treatment on cerebral ischemia. OBJECTIVE To study the treatment effect of Gastrodia elata and Uncaria rhynchophylla water extract (GUW) against cerebral ischemia, and the metabolomic profile of cerebrospinal fluid in GUW treated cerebral ischemia in rat model. METHODS Middle cerebral artery occlusion was performed in male SD rat. GUW or control (PBS) was administered orally for 7d post-operationally. At day 7, the brain of the rat was harvested. infarct volume of the brain was measured after TTC staining. The brain was subjected to H & E and cresyl violet staining in histological analysis. cerebrospinal fluid (CSF) of another set of animals was aspirated. CSF samples were labeled with 13 C-dansyl and 12C-dansyl chloride. The reaction mixture was put into liquid chromatography Fourier-transform ion cyclotron resonance mass spectrometry for analysis of the CSF metabolome. Multivariate statistical analysis will be carried out to determine the differences of metabolomic profile in CSF of GUW versus control group .RESULTS After GUW treatment, the neurological deficit score was significantly improved from day 3 to 7 (P <0.001) .GUW treated group significantly reduced 55.7% infarct volume on MCAO rats in TTC stained brain slices (P <0.001) and preserved brain histological structure observed in H & E and cresyl violet staining. On the different statistical analyzes to compare CSF metabolite in the GUW and control group, volcano plot analysis showed 48 metabolites (P <0.001); OPLS-DA analysis showed 129 metabolites (VIP score> 2.0) and 45 metabolites in ROC curve analysis. Modified identified metabolites of L-aspartic acid (60%) and glycine (62%) decreased in GUW while threoninyl-alanine increased by 1.6 fold significantly. CONCLUSION GUW ameliorated cerebral ischemic injury, and the metabolomic approach may be a potential means to find biomarker of the Chinese medicine treatment on cerebral ischemia.