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目的:了解分离自临床铜绿假单胞菌分子流行病学特性及菌株亲缘性。方法:对分离自临床的32株铜绿假单胞菌采用Kirby-Bauer法测定抗菌药物的敏感性;利用脉冲场电泳和多耐药基因聚类分析菌株的亲缘性。结果:32株铜绿假单胞菌中有17株对8种以上抗生素耐药,占53.1%,8株(25%)铜绿假单胞菌对检测抗生素全部耐药,为同一耐药表型。脉冲场电泳聚类分析显示,铜绿假单胞菌主要为3个克隆株;而多耐药基因聚类分析显示,铜绿假单胞菌主要为2个簇群,二个簇群内已发生衍化,存在3个克隆传播。结论:分离自临床的铜绿假单胞菌耐药严重,亲缘性分析中多基因聚类分析法分辨率高于脉冲场电泳。
OBJECTIVE: To understand the molecular epidemiological characteristics of isolated Pseudomonas aeruginosa and its phylogenetic relationship. Methods: Kirby-Bauer method was used to determine the susceptibility of 32 strains of Pseudomonas aeruginosa isolated from clinical isolates. The phylogenetic relationships of the isolates were analyzed by pulsed-field electrophoresis and multi-resistant gene cluster analysis. Results: Among 32 strains of Pseudomonas aeruginosa, 17 strains were resistant to more than 8 antibiotics, accounting for 53.1%. 8 strains (25%) of Pseudomonas aeruginosa were all resistant to the tested antibiotics and had the same resistant phenotype. Cluster analysis of pulsed-field electrophoresis showed that Pseudomonas aeruginosa mainly consisted of three clonal strains. Cluster analysis showed that Pseudomonas aeruginosa mainly consisted of two clusters and two clusters had been derived , There are 3 clones spread. Conclusion: The clinical isolates of Pseudomonas aeruginosa are highly resistant. The resolution of polygene cluster analysis in phylogenetic analysis is higher than that in pulsed-field electrophoresis.