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用圣堂山野生大叶韭菜的茎尖作外植体接入 MS+2 ,4 D2 mg/ L+KT1mg/ L+ZT1m g/ L 诱导培养基中 ,经过 30天培养 ,可萌生 8～ 10倍不定芽 ;在 MS+KT1mg/ L+ZT1m g/ L+NAA1m g/ L 增殖培养基培养 ,增殖系数为 5～ 10倍 ;生根培养基为 1/ 2 MS+BA0 .2 mg/ L+NAA0 .5 m g/ L,组培苗移栽成活率达 95 %。
The explants of the stems of the wild large leaf leeks of the Seongsan Mountain were used as explants and were cultured in MS + 2, 4 D2 mg / L + KT1mg / L + ZT1m g / L induction medium for 8 to 10 times Adventitious buds were cultured in MS + KT1mg / L + ZT1m g / L + NAA1m g / L proliferation medium with multiplication factor of 5 to 10 times. The rooting medium was 1/2 MS + BA0.2 mg / L + NAA0. 5 mg / L, the transplanting survival rate of tissue culture seedlings was 95%.