本研究的目的是克隆人神经营养素-4成熟蛋白基因(mhNT-4)并进行序列分析。应用PCR技术,以正常人血淋巴细胞染色体DNA为模板,扩增出人神经营养素-4(hNT-4)成熟蛋白编码基因。将所得基因片段重组于pGEM-T Easy质粒,筛选得到含人mhNT-4基因的阳性克隆。采用Sanger单链末端终止法测出全部的核苷酸序列。所得到的序列与国外文献所报道的结果(GenBank,M86528)完全相同。mhNT-4成熟蛋白基因的成功克隆,为研究其在原核细胞中的表达提供了有利条件。本文结果也提示不同人种间及不同个体间hNT-4成熟蛋白基因是相对保守的。 The purpose of this study was to clone the human neurotrophin-4 mature protein gene (mhNT-4) and perform sequence analysis. PCR technique was used to amplify the gene encoding human neurotrophin-4 (hNT-4) mature protein using the chromosomal DNA of normal human lymphocytes as a template. The resulting gene fragment was recombined in pGEM-T Easy plasmid and screened to obtain positive clones containing human mhNT-4 gene. The entire nucleotide sequence was determined using the Sanger single-stranded terminator method. The obtained sequence is exactly the same as reported in foreign literature (GenBank, M86528). The successful cloning of the mhNT-4 mature protein gene provides favorable conditions for the study of its expression in prokaryotic cells. Our results also suggest that the hNT-4 mature protein genes are relatively conserved among different species and individuals.