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丁酸钠通过影响组蛋白的乙酰化,从而影响基因表达的研究已日益受到人们的注视。本文报道用离体心脏灌流技术,以蔗糖密度梯度超速离心分离出心肌细胞核和非心肌细胞核这两种心脏细胞核,分别提取其组蛋白,从凝胶电泳以及放射自显影的结果,观察和分析了丁酸钠对~3H-乙酸基掺入大鼠心脏细胞核组蛋白的影响。结果表明,丁酸钠对组蛋白的乙酰化修饰有着明显的影响作用。两种核组蛋白被标记的程度为H_3>H_(2B)>H_4>H_2A,H_1测不出放射性;H_4形成H4_1、H4_2、H4_3和H4_4,其中以H4_1量最多,H_3产生H3_1、H3_2和H3~3,而以H3_2为最多。结果还表明,非心肌细胞的代谢转换率较心肌细胞为高。
Sodium butyrate has been gaining more and more attention by affecting the histone acetylation, which affects the gene expression. This article reports the use of isolated cardiac perfusion technology, sucrose density gradient ultracentrifugation was isolated cardiac myocytes nuclei and non-cardiac muscle nuclei of these two kinds of cardiac nuclei were extracted from the histones, gel electrophoresis and autoradiography results were observed and analyzed Effect of sodium butyrate on 3H - acetate incorporation in rat cardiac nuclei histones. The results showed that sodium butyrate had a significant effect on the acetylation of histone. The two nuclear proteins were labeled with H_3> H_ (2B)> H_4> H_2A, and H_4 did not detect radioactivity. H_4 formed H4_1, H4_2, H_4_3 and H_4_4, ~ 3, while H3_2 is the most. The results also show that non-cardiomyocytes than the metabolic rate of myocardial cells is high.