论文部分内容阅读
[目的]研究凉血化瘀方对MRL/lpr狼疮鼠蛋白尿及抗ds-DNA抗体的影响。[方法]MRL/lpr小鼠35只,饲养至3月龄。将35只雌性MRI/lpr小鼠随机分为五组,每组7只。分别为:阴性组、模型组、西药强的松加环磷酰胺组、中药加强的松加环磷酰胺组、中药组。实验小鼠均于灌胃前、灌胃21d、灌胃49d时代谢笼采集24h尿蛋白定量;眼眶后静脉丛采血,取血并分离血清后,置-70℃冰箱保存,行ELISA检测抗ds-DNA抗体的表达。[结果]MRL/lpr小鼠模型组ds-DNA水平较阴性组明显升高,蛋白尿高于阴性组。经过西药(CTX、强的松)、中药(凉血化瘀方)及中西医结合治疗(CTX、强的松、凉血化瘀方),各治疗组蛋白尿显著少于模型组。但降蛋白尿上,西药组疗效优于中药组,中西医结合组疗效优于单用中药或单用西药组,差异有统计学意义(P<0.05)。抗ds-DNA抗体血清含量模型组3.026±0.129,阴性组1.404±0.064,两组相比,P<0.01。[结论]中药凉血化瘀方具有降低血抗ds-DNA水平及蛋白尿的作用。
[Objective] To study the effect of Liangxuehuayu Recipe on proteinuria and anti-dsDNA antibody of MRL / lpr lupus mice. [Method] Thirty-five MRL / lpr mice were housed until 3 months of age. Thirty-five female MRI / lpr mice were randomly divided into five groups of seven. Respectively: negative group, model group, Western medicine prednisolone plus cyclophosphamide group, Chinese medicine to strengthen the pine plus cyclophosphamide group, traditional Chinese medicine group. The mice in the experimental group were given gavage for 21 days before gavage, and the urinary protein in 24 h after gavage was collected. Urine protein was quantitated 24 h after the gavage. The orbital venous plexus was collected for blood collection and blood separation. The serum was collected and stored in -70 ℃ refrigerator. -DNA antibody expression. [Results] The level of ds-DNA in MRL / lpr mouse model group was significantly higher than that in the negative group, and the proteinuria was higher than that in the negative group. After Western medicine (CTX, prednisone), traditional Chinese medicine (cooling blood and removing blood stasis) and Integrative Medicine (CTX, prednisone, cooling blood stasis side), each treatment group proteinuria was significantly less than the model group. However, the proteinuria, the Western medicine group was better than the traditional Chinese medicine group, the combination of traditional Chinese and Western medicine group was superior to single Chinese medicine or western medicine group, the difference was statistically significant (P <0.05). Anti-ds-DNA antibody serum content of model group 3.026 ± 0.129, negative group 1.404 ± 0.064, compared with the two groups, P <0.01. [Conclusion] The traditional Chinese medicine Liangxue Huayu Recipe has the effect of lowering blood anti-ds-DNA level and proteinuria.