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以大粒品种TD70和小粒品种Kasalath为背景材料,分别对其中的GW2和GS3基因进行了反义和超表达载体的构建和转化。结果表明,Kasalath来源的GW2(GW2~K)和GS3(GS3~K)转化TD70的超表达材料籽粒质量均降低,且GW2~K的贡献大于GS3~K;GW2~K和GS3~K转化Kasalath的反义表达材料籽粒质量均增加,GW2~K的贡献大于GS3~K;TD70来源的GW2(GW2~T)转化Kasalath的超表达材料千粒质量显著增加,而TD70来源的GS3(GS3~T)转化Kasalath的超表达材料粒型和籽粒质量没有显著变化,说明不同遗传背景中GW2和GS3的基因效应有差异,但同一背景下GW2对籽粒质量的贡献大于GS3。
Taking the large grain variety TD70 and the small grain variety Kasalath as the background material, the antisense and overexpression vectors of GW2 and GS3 genes were constructed and transformed respectively. The results showed that the grain quality of overexpression materials of GW2 (Kg2 ~ K) and GS3 (GS3 ~ K) transformed with TDK from Kasalath decreased, and the contribution of GW2 ~ K was greater than that of GS3 ~ K; GW2 ~ K and GS3 ~ K transformed Kasalath Kg2 ~ K contributed more than GS3 ~ K; TD1000 derived from GW2 (GW2 ~ T) transformed Kasalath overexpression material significantly increased the quality of 1000 grain, while the TD70-derived GS3 (GS3 ~ T ) There was no significant change in the grain size and grain quality of the over-expressed materials transformed by Kasalath, indicating that the genetic effects of GW2 and GS3 were different in different genetic backgrounds. However, under the same background, the contribution of GW2 to grain quality was greater than that of GS3.