目的了解细胞内Ｃａ２＋在三尖杉酯碱诱导ＨＬ６０细胞凋亡中的作用。方法以三尖杉酯碱单独或与Ｃａ２＋螯合剂ＥＧＴＡ共同处理ＨＬ６０细胞后，用流式细胞仪和ＤＮＡ凝胶电泳检测细胞凋亡的发生。结果Ｃａ２＋螯合剂ＥＧＴＡ抑制了细胞凋亡。结论三尖杉脂碱诱导的ＨＬ６０细胞凋亡需要Ｃａ２＋参与 Objective To understand the role of intracellular Ca2+ in harringtonine-induced apoptosis of HL60 cells. Methods HL60 cells were treated with harringtonine alone or Ca2+ chelating agent EGTA. The apoptosis was detected by flow cytometry and DNA gel electrophoresis. Results Ca2+ chelator EGTA inhibited apoptosis. Conclusions The cisplatin-induced apoptosis of HL60 cells requires Ca2+ involvement