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Drought is one of the most significant abiotic stresses that influence plant growth anddevelopment.Expression analysis revealed that OsNRT1.3,a putative nitrate transporter gene in rice,wasinduced by drought.To confirm if the OsNRT1.3 promoter can respond to drought stress,a 2019 bpupstream sequence of OsNRT1.3 was cloned.Three OsNRT1.3 promoter fragments were generated by5′-deletion,and fused to the β-glucuronidase (GUS) gene.The chimeric genes were introduced into riceplants.NRT2019::GUS,NRT1196::GUS and NRT719::GUS showed similar expression patterns in seeds,roots,leaves and flowers in all transgenic rice,and GUS activity conferred by different OsNRT1.3 promoterfragments was significantly upregulated by drought stress,indicating that OsNRT1.3 promoter responds todrought stress and the 719 bp upstream sequence of OsNRT1.3 contains the drought response elements.
Drought is one of the most significant abiotic stresses that influence plant growth and development. Expression Analysis revealed that OsNRT1.3, a putative nitrate transporter gene in rice, wasinduced by drought. To confirm if the OsNRT1.3 promoter can respond to drought stress, a 2019 bpupstream sequence of OsNRT1.3 was cloned.Three OsNRT1.3 promoter fragments were generated by 5’-deletion, and fused to the β-glucuronidase (GUS) gene.The chimeric genes were introduced into rice plants. NRT2019 :: GUS, NRT1196: : GUS and NRT719 :: GUS showed similar expression patterns in seeds, roots, leaves and flowers in all transgenic rice, and GUS activity conferred by different OsNRT1.3 promoterfragments were significantly upregulated by drought stress, indicating that OsNRT1.3 promoter responds todrought stress and the 719 bp upstream sequence of OsNRT1.3 contains the drought response elements.