论文部分内容阅读
人载脂蛋白B mRNA编辑酶催化多肽样蛋白3G(apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3protein G,APOBEC3G)是宿主的抗HIV-1(human immunodeficiency virus type 1)因子,而HIV-1辅助蛋白———病毒感染因子Vif(viral infectivity factor)可通过介导蛋白酶体途径降解APOBEC3G,因此针对APOBEC3G及HIV-1Vif进行抑制剂设计已经成为抗HIV-1药物研究新的方向之一,相应用于研究Vif-APOBEC3G相互作用的方法也越来越多,如免疫印迹、免疫杂交、脉冲追踪试验、生物发光共振能量转移检测、BIAcore检测等。作者将目前用于以Vif-APOBEC3G为靶点的药物的筛选及作用机制的研究方法进行了综述,为基于此的研究提供了策略。
Human apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3 protein G (APOBEC3G) is a host anti-HIV-1 (human immunodeficiency virus type 1) factor, whereas HIV-1 accessory protein- - Viral viral factor Vif degrades APOBEC3G through the proteasomal pathway. Therefore, the design of inhibitors targeting APOBEC3G and HIV-1Vif has become one of the new directions in the research of anti-HIV-1 drugs, Vif-APOBEC3G interaction methods are also more and more, such as Western blotting, immune hybridization, pulse tracing experiments, bioluminescence resonance energy transfer detection, BIAcore detection. This review summarizes the current research methods for the screening and mechanism of drugs targeting Vif-APOBEC3G, which provides a strategy for the research based on this.