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目的:建立测定健胃止痛五味胶囊中胡椒碱含量的HPLC法。方法:采用美国AlltechRP-C18(5,4.6mm×250mm)色谱柱,以甲醇-水(77∶23)为流动相,检测波长343nm,流速1.0mL/min,柱温30℃。结果:胡椒碱的平均回收率为99.54%(RSD为1.7%)。结论:用本法测定健胃止痛五味胶囊中胡椒碱含量准确可靠。
Objective: To establish an HPLC method for the determination of piperine in Jianwei Zhitong Wuwei Capsule. Methods: Alltech RP-C18 column (5,4.6 mm × 250 mm) was used in this study. The mobile phase consisted of methanol and water (77:23). The detection wavelength was set at 343 nm and the flow rate was set at 1.0 mL / min. Results: The average recovery of piperine was 99.54% (RSD 1.7%). Conclusion: This method is accurate and reliable for the determination of piperine in Jianwei Zhitong Wuwei Capsule.