本实验用皮质激素类药物、4℃贮存和紫外线照射分别于体外处理小鼠跖部皮肤然后,1.经ATP_(ase)方法显示Langerhans细胞,观察其形态变化和测定细胞内ATP_(ase)含量。2.电镜观察其超微结构的变化。3.异体皮肤移植观察其存活期的变化。结果,异体皮肤在体外受到理化因素怍用后,其表皮Langerhans细胞受到不同程度的损伤;经异体移植后,存活期明显延长。 In this study, cortical hormone drugs, 4 ℃ storage and UV irradiation were treated in vitro plantar skin of mice and then, by the ATP ase method Langerhans cells were observed morphological changes and determination of intracellular ATP ase content . Electron microscopy observation of its ultrastructure changes. 3. Allograft skin graft survival changes observed. As a result, the allogeneic skin was subjected to physical and chemical factors in vitro, and the Langerhans cells in the epidermis were damaged to different extents. After allogeneic transplantation, the survival period was significantly prolonged.