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目的优化人乳中甲硝唑的检测,建立限度检查方法。方法用均匀设计法确定试验方案,优化从人乳中提取甲硝唑的条件,用HPLC测定甲硝唑。结果从人乳中提取甲硝唑,不需要加氢氧化钠,也可以不进行超声处理。限量检测方法的验证试验结果,空白样品色谱图上在与供试品和对照品色谱相应位置处没有色谱峰;检测限<0.02μg·L-1;在不同条件下检测同一样品,结果RSD为2.2%。结论用均匀设计法可以迅速有效地确定检测方法的前处理条件。建立的人乳中甲硝唑限度检测方法灵敏度高、操作简单、分析速度快、重复性好。
Objective To optimize the detection of metronidazole in human milk and establish the limit test method. Methods The experimental design was determined by uniform design method, the conditions of extracting metronidazole from human milk were optimized, and metronidazole was determined by HPLC. Results Metronidazole was extracted from human milk without the addition of sodium hydroxide and was not sonicated. The limit test method validation test results, the blank sample chromatogram at the corresponding position with the test sample and reference substance chromatography no peak; detection limit <0.02μg · L-1; under the same conditions in the same sample test, the results RSD 2.2%. Conclusion The uniform design method can quickly and effectively determine the pretreatment conditions of detection methods. The metronidazole limit detection method established in human milk has high sensitivity, simple operation, fast analysis and good repeatability.