论文部分内容阅读
目的 :建立HPLC法测定口腔溃疡糊剂中盐酸金霉素含量。方法 :色谱柱为KF C18柱 ( 4 .6mm× 2 5 0mm ,10 μm) ;柱温 :4 0℃ ;流动相为水 N ,N二甲基甲酰胺 -乙腈 ( 6 8∶2 7∶5 ) ,含磷酸氢二钠 0 .0 0 6 3mol·L-1和乙二胺四乙酸二钠 0 .0 1mol·L-1,并以 10mol·L-1氢氧化钠溶液调节pH至 8.5 ;检测波长为 380nm。结果 :盐酸金霉素在 10～ 10 0 μg·ml-1的范围内线性关系良好 ,r =0 .9996 ,平均回收率为 99.4 % ,RSD为 1.4 %。结论 :本方法简便 ,快速 ,准确 ,可用于口腔溃疡糊剂的质量控制
Objective: To establish an HPLC method for the determination of chlortetracycline hydrochloride in oral ulcer paste. Methods: The column was KF C18 column (4.6mm × 250mm, 10μm); the column temperature was 40 ℃; the mobile phase was water N, N-dimethylformamide-acetonitrile ) Containing 0,06,3 mol·L-1 of disodium hydrogen phosphate and 0,01 mol·L-1 of disodium ethylenediaminetetraacetate, and the pH was adjusted to 8.5 with 10 mol·L-1 sodium hydroxide solution. The detection wavelength is 380nm. Results: Chlortetracycline hydrochloride showed a good linearity in the range of 10 ~ 100 μg · ml-1 with r = 0.9996, with an average recovery of 99.4% and a RSD of 1.4%. Conclusion: The method is simple, rapid and accurate and can be used for the quality control of oral ulcer paste