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目的研究(HCV)E2/NS1相对保守区多肽抗原在检测抗-HCV中的意义。方法利用HCVE2/NS1基因编码的膜区糖蛋白合成E2/NS1相对保守区多肽抗原,建立酶免疫试验(EIA),对96例HCV感染者及40例正常献血员进行抗HCVE2/NS1抗体的检测,同时平行检测HCVRNA和丙氨酸转氨酶(ALT)。结果抗HCVE2/NS1在HCV感染者的检出率为41.67%,其中急性丙型肝炎为13.55%,慢性丙型肝炎为25.04%,无症状感染者为2.08%;正常献血员中发现3例抗HCVE2/NS1抗体弱阳性,而HCVRNA阴性;对抗HCVE2/NS1与HCVRNA比较,两者的共同阳性率为62.5%,抗HCVE2/NS1与HCVRNA具有相关性;在抗HCVE2/NS1阳性的急慢性患者中ALT值之间有明显差异(P<0.05),急性比慢性高;抗HCVE2/NS1与RNA共同阳性率中慢性感染明显高于急性感染。结论抗HCVE2/NS1阳性同时也有病毒核酸存在,但抗HCVE2/NS1相对保守区抗体可能有一定的中和作用,这种体液免疫在清除HCV感染中有一定的作用,抗HCVE2/NS1相对保守区抗体是否就是中和抗体仍有待进一步研究?
Objective To study the significance of HCV E2 / NS1 polypeptide antigen in the detection of anti-HCV. Methods Enzyme linked immunosorbent assay (EIA) was used to synthesize E2 / NS1 polypeptide antigen from the membrane glycoprotein encoded by HCVE2 / NS1 gene. Anti-HCVE2 / NS1 antibodies were detected in 96 HCV-infected and 40 normal donors , While parallel detection of HCV RNA and alanine aminotransferase (ALT). Results The detection rate of anti - HCVE2 / NS1 in HCV infection was 41.67%, of which, acute hepatitis C was 13.55%, chronic hepatitis C was 25.04% and asymptomatic infection was 2.08%. In the normal blood donors, three anti-HCVE2 / NS1 antibodies were found to be weakly positive and negative for HCVRNA. The co-positive rate of anti-HCVE2 / NS1 and HCVRNA was 62.5% ALT values were significantly different between acute and chronic HCVE2 / NS1-positive patients (P <0.05), acute and chronic. The positive rate of anti-HCVE2 / NS1 and RNA was significantly higher than that of acute infection. Conclusions Anti-HCVE2 / NS1 positivity also exists in the presence of viral nucleic acid. However, the antibody against HCVE2 / NS1 may have a certain neutralization effect. This humoral immunity may play a role in the clearance of HCV infection, and the relative conserved region of anti-HCVE2 / NS1 Whether the antibody is neutralizing antibody remains to be further studied?