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作者应用再改良的悬滴培养方法，培养鸡胚背根节。将Hamburger35期鸡胚腰段背很节分别种植于涂有鼠尾胶原的48孔组织培养板孔中，每扎加入培养液100VI，翻转培养板，使背根节和培养液滴悬挂在培养孔内。再将培养板置入加有一定量Hanks平＠I盐溶液的玻璃缸中，密封缸周绿，将其放入37C恒温箱中培养。利用NaHCO。和H对的作用能释放CO。以及水份受热蒸发产生相对湿度，从而在缸内建立相当CO。孵箱的细胞生长环境。本方法是在本研究组改良悬滴培养法的基础上作了进一步改进，改进后的方法操作更为简便，更能节约大量人力、物力和时间，并且由于每批各实验组间所培养的背根节都生长在同一培养板的环境中，保证了实验的同步及培养条件的完全一致。我们应用本法进行了二十余次实验，共种植了1000余个背根节，对经凝胶过滤层析和高效液相色谱技术分离的备用背根描脊髓背角提取液各组份进行了生物学活性检测，确定了有活性的组份及其有效作用的最低剂量。结果表明本方法是体外培养鸡胚背报节的一种简便可靠、省时、省力的好方法。
The authors then use improved hanging drop culture method to cultivate chicken embryo dorsal root ganglia. The Hamburger 35 chicken embryo lumbar segment were implanted in the sections were stained with rat tail collagen 48-well tissue culture plate wells, each culture solution was added 100VI bar, turn the plate, the dorsal root ganglion and culture suspension hanging in the culture hole Inside. The plates were then placed in a glass jar filled with a quantity of Hanks’s salt solution and the jar was sealed around the green, which was then incubated in a 37C incubator. Using NaHCO. The role of H pairs releases CO. And the water is heated and evaporated to generate relative humidity, thus establishing a comparable CO in the cylinder. Incubator cell growth environment. The method is further improved on the basis of the improved hanging drop culture method in the research group, and the improved method operation is more simple and convenient, more manpower, material and time can be saved, and because each batch of experimental groups is cultured Dorsal root ganglia are grown in the same culture plate environment, to ensure the synchronization of experiments and culture conditions are exactly the same. We applied this method for more than 20 experiments, planting a total of more than 1,000 dorsal root segments, by gel filtration chromatography and high performance liquid chromatography separation of the dorsal root spinous roots back extract of each component The biological activity test to determine the active ingredients and the minimum effective dose. The results show that this method is a simple, reliable, time-saving and labor-saving method for culturing chick embryo in vitro.