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用改良 ASG法在黑麦 ( Secale cereale)有丝分裂早中期、中期对染色体进行了 G-带分析 ,并作了G-带带型和变动性分析。结果表明 ,无论是早中期还是中期 ,黑麦染色体都显示了清晰的 G-带 ,而且带纹数目多、细窄而大小较相近 ,分布较密集而均匀 ;同源染色体带纹的数目、分布位置基本一致 ,可较为准确地配对。随着分裂时期的推进 ,G-带带数减少 ,从早中期至中期单倍染色体组 G-带带数减少了 2 9.0 %,单倍染色体组的长度缩短了 2 5.0 %,带纹减少幅度与染色体长度缩短幅度相近。对黑麦 G-带的特征与变动性、G-显带技术及其应用等问题进行了讨论
The improved ASG method was used to analyze the G-banding of chromosomes in early, middle and mid mitosis in Secale cereale and to analyze the G-banding and variability. The results showed that the chromosomes of rye showed a clear G-band in early, middle and mid-term, and the number of bands was more, the number of bands was smaller, the size was narrower, the size was similar, and the distribution was dense and uniform. The number and distribution of bands of homologous chromosomes Basically the same location, can be more accurately match. With the advancing of the split period, the number of G-band decreased, the number of G-band in hapyterase decreased by 9.09%, the length of haploid chromosome decreased by 5.0% Similar to the shortening of chromosome length. The characteristics and variability of G-banding of rye, G-banding technique and its application are discussed