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为掌握贵州省柑桔黄龙病的疫情及了解病原菌的遗传多样性,2015—2016年在15个县(市)采集了104份柑桔黄龙病疑似样品,进行柑桔黄龙病菌16SrDNA常规PCR检测及柑桔黄龙病菌亚洲种噬菌体转移酶基因位点多态性分析。结果表明,供试样品有19份检测为阳性,阳性检出率为18.27%。贵州柑桔黄龙病疫情主要集中在罗甸、望谟、兴义、册亨等黔南和黔西南柑桔黄龙病发生老区,从江、榕江等黔东南地区疫情得到一定控制,遵义及铜仁等地区未发现疫情。贵州省内柑桔黄龙病菌有丰富的遗传多态性,在地理来源及寄主品种来源上均表现出遗传差异。
To understand the epidemic situation of citrus Huanglongbing in Guizhou Province and to understand the genetic diversity of the pathogenic bacteria, 104 suspected citrus Huanglongbing samples were collected in 15 counties (cities) from 2015 to 2016, and 16S rDNA of common citrus Analysis of Asian Bacteriophage Transferase Gene Polymorphism in Citrus Huanglongbing. The results showed that 19 samples tested positive, the positive detection rate was 18.27%. Guizhou Citrus Huanglongbing epidemic mainly in Luodian, Wang mo, Xingyi, such as the southern city of Qianheng and Qianlong citrus Huanglongbing old areas, Congjiang, Rongjiang and other Southeast Guizhou epidemic outbreaks have been controlled, Zunyi and Tongren No other outbreaks were found in other areas. There is abundant genetic polymorphism of citrus yellow dragon in Guizhou Province, showing genetic difference in geographical origin and host variety.