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目的:观察CD44+/ki-67-结直肠癌干细胞的特征及其与临床病理特征的关系.方法:应用免疫组织化学染色、免疫组织化学双重染色和苏木精-伊红(HE)染色,检测10例正常肠黏膜,15例腺瘤,59例结直肠癌标本和人结肠癌细胞株SW620中CD44和ki-67的表达,定位CD44+/ki-67-肿瘤细胞,观察、计数并与对应的HE染色切片进行形态学比较,讨论CD44+/ki-67-癌干细胞与其临床病理特征的关系.结果:CD44+/ki-67-肿瘤细胞的数量占肿瘤细胞0.1%-25.0%,平均5.82%,这种细胞主要分布在腺体基底膜侧或者在共壁腺体的共壁侧,呈散在分布,核的形态较一致,呈圆形或卵圆形,染色质较深,胞浆较少,与正常肠黏膜隐窝底部干细胞形态较一致,与肿瘤浸润深度(χ2=1.851,P<0.05)和是否有淋巴结转移相关(χ2=-4.113,P<0.01).结论:CD44+/ki-67-可以作为肿瘤干细胞的标志,这为肿瘤干细胞的分离、靶向治疗和个体化治疗提供了可靠的分子生物学指标,也是预测肿瘤转移和判断患者预后的可靠指标.
Objective: To observe the characteristics of CD44 + / ki-67-colorectal cancer stem cells and its relationship with clinicopathological features.Methods: Immunohistochemical staining, immunohistochemical staining and hematoxylin-eosin (HE) 10 cases of normal intestinal mucosa, 15 cases of adenoma, 59 cases of colorectal cancer specimens and human colon cancer cell line SW620 CD44 and ki-67 expression, localization of CD44 + / ki-67- tumor cells observed and counted and with the corresponding HE staining sections were used to compare the morphological features of CD44 + / ki-67-cancer cells with clinicopathological features.Results: The number of CD44 + / ki-67- tumor cells accounted for 0.1% -25.0% of tumor cells with an average of 5.82% Species of cells are mainly distributed in the basement membrane of the gland or co-wall side of the co-wall gland, scattered distribution, nuclear morphology is more consistent, round or oval, darker chromatin, less cytoplasm, and The shape of stem cells at the bottom of normal crypt mucosa was consistent with the depth of invasion (χ2 = 1.851, P <0.05) and lymph node metastasis (χ2 = -4.113, P <0.01) .Conclusion: CD44 + / ki-67- As a marker for cancer stem cells, this provides a reliable basis for the isolation, targeted therapy and individualized treatment of tumor stem cells Sub biological indicators, and determines tumor metastasis prediction is a reliable indicator of prognosis.