利用脂肪酶在非水相体系中催化的对映选择性酯化反应，对外消旋手性药物中间体（±）－１－氯－３－（１－萘氧）－２－丙醇（简称萘氧氯丙醇）进行了拆分，考察了溶剂、酰化剂和底物浓度对酶活性和选择性的影响。结果表明，当溶剂为异辛烷，酰化剂为醋酸酐、底物浓度为氯醇１００ｍｍｏｌ／Ｌ、酸酐５０ｍｍｏｌ／Ｌ时，酶显示出最佳的催化活性和选择性，产物酯的光学纯度可达９０％。 Using the enantioselective esterification catalyzed by lipase in non-aqueous system, the racemic chiral drug intermediate (±) -1-chloro-3- (1-naphthalenedioxy) Naphthalene oxychloropropanol) were resolved, investigated the solvent, acylating agent and substrate concentration on the activity and selectivity. The results showed that when the solvent is isooctane, the acylating agent is acetic anhydride, the substrate concentration is 100 mmol / L chlorohydrin and the acid anhydride is 50 mmol / L, the enzyme shows the best catalytic activity and selectivity. The optical purity Up to 90%.