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研究以 5 7份中美大豆抗胞囊线虫病种质资源为实验材料 ,利用基于检测微珠的单碱基延伸方法 ,对与大豆胞囊线虫病 (SCN)抗性基因rhg1和Rhg4紧密连锁的SNPs进行分析 ,目的是阐明我国大豆抗性种质在这两个位点的SNPs等位变异分布频率 ,为中国大豆种质抗SCN资源的利用奠定基础。分析结果表明 ,SNPs的抗性等位基因与中国大豆种质综合抗性的关系比不同生理小种的抗性关系更为密切。在rhg1和Rhg4位点 ,美国的 9份抗性种质中 ,有 7份抗性种质的SNPs均为纯合抗病基因型 ,而中国 4 8份抗性种质中有 32份 ,分别占鉴定总数的 77 8%和 6 6 7% ,推测大豆抗SCN种质中 ,以rhg1和Rhg4这两个基因协同作用表现出的抗性可能占多数 ,但还存在其他的抗性机制。
In this study, 57 germplasm resources of soybean cyst nematode resistance were used as experimental materials, and single-base extension method based on the detection of microspheres was used to study the resistance to soybean cyst nematode (SCN) resistance genes rhg1 and Rhg4 The purpose of this study was to elucidate the frequency of allelic variation of SNPs in soybean germplasm at these two loci in China so as to lay a foundation for the utilization of SCN resources in Chinese soybean germplasm. The results showed that the relationship between the resistance allele of SNPs and the comprehensive resistance of soybean germplasm in China was more closely related to the resistance of different physiological races. Among the 9 resistant germplasms in rhg1 and rhg4, 7 of the resistant germplasms were homozygous resistant, while 32 of the 48 resistant germplasms in China Accounting for 77.8% and 66.7% of the total identified. It is speculated that the resistance of syngeneic rhg1 and Rhg4 may be the majority in soybean anti-SCN germplasm, but other resistance mechanisms exist.