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本文对自德国土壤中分离的８株苏云金芽孢杆菌进行毒力测定和晶体蛋白特性的研究。生物测定结果表明，８株Ｂｔ中除Ｓ２１－１、Ｓ２１－１３对甜菜夜蛾无毒性外，对夜蛾科的４种供试昆虫均有很高的毒效，４８小时校正死亡率高达８０％～１００％；扫描电镜观察和ＳＤＳ－ＰＡＧＥ晶体蛋白电泳结果可知，Ｓ１１－６和Ｓ２１－１３２个菌株只有一种菱形晶体和一条相应的１３５ＫＤａ主带，其余６个菌株均有菱形和方形两种晶体，以及两条相应的１３５ＫＤａ和６５ＫＤａ主带。另对晶体的特性与其毒力的关系进行了讨论。
In this paper, eight strains of B. thuringiensis isolated from soil in Germany were studied for their virulence and crystal proteins. The bioassay results showed that the eight Bt strains except S21-1 and S21-13 were non-toxic to beet armyworm and had high toxic effect on four tested insects of the noctuid family. The 48-hour corrected mortality rate was as high as 80 % ~ 100%. Scanning electron microscopy and SDS-PAGE crystal protein electrophoresis showed that there were only one diamond crystal and one corresponding 135KDa main band in S11-6 and S21-132 strains, and the other six strains had rhombus and square two Seed crystals, and two corresponding 135KDa and 65KDa main bands. The relationship between the characteristics of crystals and their virulence is also discussed.