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目的:探讨利开灵对脑出血后大鼠体内继发肠源性内毒素血症的干预作用。方法:采用Rosenberg法改良后建立大鼠脑出血模型,根据脑出血不同时点(12h、24h、48h和72h)分为4组,每个时间点再分为3组:假手术组、模型组、利开灵组,观察利开灵对肠组织相应病理改变的影响,并用终点显色法检测各组血浆内毒素(LPS)水平,ELISA法检测各组血清TNF-α、IL-1β水平。结果:模型组血浆LPS各时点均明显升高,在48h达高峰(P<0.01);血清TNF-α、IL-1β升高,均在24h达高峰(P<0.01)。同时发现利开灵可明显改善肠组织病理形态损伤,下调血浆LPS以及血清TNF-α、IL-1β过高表达。结论:脑出血大鼠继发肠源性内毒素血症,利开灵对其有较好的调控作用,这对改善脑出血预后具有积极意义。
Objective: To investigate the intervention effect of LiKailing on the secondary intestinal endotoxemia in rats after intracerebral hemorrhage. Methods: The model of intracerebral hemorrhage was established by Rosenberg method and divided into 4 groups at different time points (12h, 24h, 48h and 72h). Each time point was further divided into 3 groups: sham operation group, model group , Likailing group to observe the effects of LiKailing on the pathological changes of intestinal tissue. Plasma endotoxin (LPS) levels in each group were measured by end-point colorimetric assay. Serum levels of TNF-α and IL-1β were detected by ELISA. Results: The levels of plasma LPS in model group were significantly increased at each time point, reaching the peak at 48h (P <0.01). The levels of TNF-α and IL-1β in serum of model group were all higher than those of control group (P <0.01). At the same time, LiKailing can significantly improve pathological damage of intestinal tissue, down-regulate plasma LPS and over-expression of serum TNF-α and IL-1β. CONCLUSION: Secondary intestinal endotoxemia is induced by intracerebral hemorrhage in rats, and Kaikailing has a good regulatory effect on it, which is of positive significance for improving the prognosis of intracerebral hemorrhage.