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目的：建立人 Ｂ淋巴瘤裸鼠移植瘤腹腔模型，主要是为了研究抗 ＣＤ２０／ＣＤ３双功能抗体对人 Ｂ淋巴瘤的治疗作用。方法：采用５周龄左右的雌性ＢＡＬＢ／ｃ－ｎｕ，腹腔注射ｐｒｉｓｔａｎｅ ０．２ｍｌ／只 ２次，经６０Ｃｏ照射，３天后腹腔接种人Ｂ淋巴瘤细胞株（Ｒａｊｉ）１×１０～７／只，并于第 ２天随机分组用预先激活的Ｔ细胞加入双功能抗体进行治疗，每周腹腔给药 １次，共４次。结果：人Ｂ淋巴瘤细胞株（Ｒａｊｉ）裸鼠腹腔移植，接种３８只均获成功，成瘤率１００％。染色体检查证明为人类染色体特征，病理形态学观察，瘤块主要分布于腹腔、肠系膜、淋巴结、贴近肠壁处，并且ＣＤ１９、ＣＤ２０，ＨＬＡ—ＤＲ阳性率达９５％以上，对照组４０天时全部死亡，治疗组存活至１００天处死。结论：成功地建立了人类Ｂ淋巴瘤裸鼠移植瘤腹腔模型，经过实验证明，抗ＣＤ３／抗 ＣＤ２０。双功能抗体有很好地抑癌作用。
OBJECTIVE: To establish an intraperitoneal model of human B-lymphoma xenograft in nude mice, mainly to study the therapeutic effect of anti-CD20/CD3 dual antibody on human B-lymphoma. METHODS: Female BALB/c-nu, 5 weeks old, was injected intraperitoneally with 0.2 ml of pristane twice, and irradiated with 60Co. Three days later, intraperitoneal injection of human B lymphoma cell line (Raji) 1×10 ～ 7/ On the second day, they were randomly divided into two groups: pre-activated T cells and bifunctional antibodies. Each week, they were administered intraperitoneally once a day for 4 times. Results: Transplantation of human B lymphoma cell line (Raji) in nude mice and 38 inoculations were all successful. The tumor formation rate was 100%. Chromosome examination proved human chromosome characteristics, pathological observation, tumor mass mainly distributed in the abdominal cavity, mesentery, lymph nodes, close to the intestinal wall, and the CD19, CD20, HLA-DR positive rate of 95% or more, the control group all died at 40 days The treatment group survived to 100 days of execution. Conclusion: The abdominal cavity model of human B-lymphoma xenograft in nude mice was successfully established. It was proved by experiments that anti-CD3/anti-CD20. Bifunctional antibodies have a good anti-cancer effect.