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长雄野生稻(O.longistaminata)起源于非洲,具有和亚洲栽培稻相同的AA基因组,是栽培稻改良的重要遗传资源。本研究构建了长雄野生稻全基因组fosmid文库,共获得33.6万克隆,文库的平均插入片段大小约为40kb,其中94.7%的克隆插入片段大小在30~50kb之间。挑取其中约11万克隆并保存,可覆盖10倍栽培稻基因组,对任意基因或序列的筛选概率达到了99.99%。将剩余的22万个克隆进行了末端配对测序,显示其有助于长雄野生稻全基因组测序的拼接。所构建的fosmid文库为筛选和克隆长雄野生稻的有利基因提供了研究材料。
O. longistaminata originates in Africa and has the same AA genome as Asian cultivated rice, which is an important genetic resource for the improvement of cultivated rice. In this study, we constructed a genome-wide fosmid library of Chang-xiong wild rice with a total of 336,000 clones. The average insert size of the library was about 40kb, of which 94.7% had a size of 30-50kb. Approximately 110,000 clones were picked and preserved to cover 10-fold of the cultivated rice genome. The screening probability of any gene or sequence was 99.99%. The remaining 220,000 clones were subjected to end-paired sequencing, which showed that they contribute to the splicing of genome-wide sequencing of long male wild rice. The constructed fosmid library provided the research materials for screening and cloning the favorable gene of Changxiong wild rice.