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选择ＳＤ大鼠切牙分离制备未成熟的牙釉质组织。参照Ｔｅｒｍｉｎｅ等的方法，采用盐酸胍及盐酸胍－ＥＤＴＡ分步提取法将牙釉质蛋白分为釉原蛋白和釉蛋白两大组分。经Ｓｅｐｈａｄｅｘ－Ｇ２００层析，初步将釉原蛋白分为Ａ１，Ａ２，Ａ３三个组分，将釉蛋白分为Ｅ１，Ｅ２两个组分。经ＳＤＳ－ＰＡＧＥ分析，所纯化之釉原蛋白Ａ１以２３ｋＤ为主要成分。在釉蛋白的层析主要分离组分Ｅ１中，蛋白含量极低，ＳＤＳ－ＰＡＧＥ测得其为４４ｋＤ的单一组分。釉质蛋白质在特定ｐＨ值，离子强度及溶液蛋白浓度改变后出现自我聚集沉淀。ＰＡＧＥ电泳分析提示，釉原蛋白分子中可能含有较多亚基。
Induction of Immature Enamel Tissues by Selecting Incisors of SD Rats. Reference to Termine and other methods, the use of guanidine hydrochloride and guanidine hydrochloride-EDTA step-by-step extraction of enamel protein into two components of amelogenin and amelogenin. Sephadex-G200 chromatography, the initial amelogenin into A1, A2, A3 three components, the amelogenin E1, E2 two components. After SDS-PAGE analysis, the purified amelogenin A1 23kD as the main component. In the main separation fraction E1 of the amelogenin chromatography, the protein content was very low, and it was a single component of 44 kD as measured by SDS-PAGE. Enamel proteins appear to self-aggregate and precipitate after a change of specific pH, ionic strength and solution protein concentration. PAGE electrophoresis analysis suggests that amelogenin molecules may contain more subunits.